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 | Acceso al texto completo restringido a Biblioteca INIA Tacuarembó. Por información adicional contacte bibliotb@tb.inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha : |
30/05/2019 |
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Actualizado : |
04/05/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
WALLACE, F.; BENNADJI, Z.; FERREIRA, F.; OLIVARO, C. |
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Afiliación : |
FEDERICO WALLACE, Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR, Tacuarembó, Uruguay.; ZOHRA BENNADJI SOUALHIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO AMARY FERREIRA CHIESA, Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR, Tacuarembó, Uruguay; Laboratorio de Carbohidratos y Glicoconjugados, Departamento de Química Orgánica, Facultad de Química, Udelar, Instituto de Higiene, Uruguay.; CRISTINA OLIVARO, Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR, Tacuarembó, Uruguay. |
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Título : |
Structural characterisation of new immunoadjuvant saponins from leaves and the first study of saponins from the bark of Quillaja brasiliensis by liquid chromatography electrospray ionisation ion trap mass spectrometry.
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Phytochemical Analysis, 1 November 2019, Volume 30, Issue 6, Pages 644-652. Doi: https://doi.org/10.1002/pca.2837 |
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DOI : |
10.1002/pca.2837 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 19 December 2018; Revised: 21 February 2019; Accepted: 27 March 2019. ACKNOWLEDGEMENTS: This work was supported by the INIA (award L4?FO?21?0?00);
the MEC (Uruguay, Fondo Vaz Ferreira fvf_2017_158) and the PEDECIBA-Química. |
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Contenido : |
Abstract. Introduction: Quillaja brasiliensis (St. A. ?Hil. & Tul) Mart (Quillajaceae) is a species native to South America, which is rich in saponins. Saponins are used in different industries, so there is a constant demand for this type of compound. Based on the wide range of applications for the saponins found in this species, notably as
immunoadjuvants, we conducted a comprehensive study of this tree and its saponins. Objective: The purpose of this work is to complete the characterisation of
the immunoadjuvant saponin fraction from Q. brasiliensis leaves and further study the saponin fraction obtained from Q. brasiliensis bark. Methodology: Saponin fractions were studied using mass spectrometry in combination with classical methods of monosaccharide and methylation analysis. We performed direct infusion and liquid chromatography/electrospray ionisation ion trap multiple?stage mass spectrometry (DI?ESI?IT?MSn and LC?ESI?IT?MS2). Results: Seventy?five saponins, 21 from leaves and 54 from bark, were tentatively identified according to their molecular mass, fragmentation pattern and chromatographic behaviour. This work represents the first investigation of saponins from the bark of Q. brasiliensis and some of them presented new structural motifs not previously reported in the genus Quillaja. Conclusion: The efficiency and selectivity of the data dependent LC?MS2 method allowed the rapid profiling of saponins from Q. brasiliensis. The results of the monosaccharide and methylation analysis performed in saponins from Q. brasiliensis fractions and Q. saponaria Molina (Quillajaceae) fraction gives further support to the structures proposed according to the mass spectral data, validating the strategy used in the present work. MenosAbstract. Introduction: Quillaja brasiliensis (St. A. ?Hil. & Tul) Mart (Quillajaceae) is a species native to South America, which is rich in saponins. Saponins are used in different industries, so there is a constant demand for this type of compound. Based on the wide range of applications for the saponins found in this species, notably as
immunoadjuvants, we conducted a comprehensive study of this tree and its saponins. Objective: The purpose of this work is to complete the characterisation of
the immunoadjuvant saponin fraction from Q. brasiliensis leaves and further study the saponin fraction obtained from Q. brasiliensis bark. Methodology: Saponin fractions were studied using mass spectrometry in combination with classical methods of monosaccharide and methylation analysis. We performed direct infusion and liquid chromatography/electrospray ionisation ion trap multiple?stage mass spectrometry (DI?ESI?IT?MSn and LC?ESI?IT?MS2). Results: Seventy?five saponins, 21 from leaves and 54 from bark, were tentatively identified according to their molecular mass, fragmentation pattern and chromatographic behaviour. This work represents the first investigation of saponins from the bark of Q. brasiliensis and some of them presented new structural motifs not previously reported in the genus Quillaja. Conclusion: The efficiency and selectivity of the data dependent LC?MS2 method allowed the rapid profiling of saponins from Q. brasiliensis. The results of the monosaccharide and methyla... Presentar Todo |
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Palabras claves : |
IMMUNOADJUVANT SAPONINS; MASS SPECTROMETRY; METHYLATION ANALYSIS; MONOSACCHARIDE ANALYIS; QUILLAJA BRASILIENSIS. |
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Asunto categoría : |
K11 Ingeniería forestal |
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Marc : |
LEADER 02910naa a2200241 a 4500
001 1059792
005 2020-05-04
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1002/pca.2837$2DOI
100 1 $aWALLACE, F.
245 $aStructural characterisation of new immunoadjuvant saponins from leaves and the first study of saponins from the bark of Quillaja brasiliensis by liquid chromatography electrospray ionisation ion trap mass spectrometry. .$h[electronic resource]
260 $c2019
500 $aArticle history: Received: 19 December 2018; Revised: 21 February 2019; Accepted: 27 March 2019. ACKNOWLEDGEMENTS: This work was supported by the INIA (award L4?FO?21?0?00); the MEC (Uruguay, Fondo Vaz Ferreira fvf_2017_158) and the PEDECIBA-Química.
520 $aAbstract. Introduction: Quillaja brasiliensis (St. A. ?Hil. & Tul) Mart (Quillajaceae) is a species native to South America, which is rich in saponins. Saponins are used in different industries, so there is a constant demand for this type of compound. Based on the wide range of applications for the saponins found in this species, notably as immunoadjuvants, we conducted a comprehensive study of this tree and its saponins. Objective: The purpose of this work is to complete the characterisation of the immunoadjuvant saponin fraction from Q. brasiliensis leaves and further study the saponin fraction obtained from Q. brasiliensis bark. Methodology: Saponin fractions were studied using mass spectrometry in combination with classical methods of monosaccharide and methylation analysis. We performed direct infusion and liquid chromatography/electrospray ionisation ion trap multiple?stage mass spectrometry (DI?ESI?IT?MSn and LC?ESI?IT?MS2). Results: Seventy?five saponins, 21 from leaves and 54 from bark, were tentatively identified according to their molecular mass, fragmentation pattern and chromatographic behaviour. This work represents the first investigation of saponins from the bark of Q. brasiliensis and some of them presented new structural motifs not previously reported in the genus Quillaja. Conclusion: The efficiency and selectivity of the data dependent LC?MS2 method allowed the rapid profiling of saponins from Q. brasiliensis. The results of the monosaccharide and methylation analysis performed in saponins from Q. brasiliensis fractions and Q. saponaria Molina (Quillajaceae) fraction gives further support to the structures proposed according to the mass spectral data, validating the strategy used in the present work.
653 $aIMMUNOADJUVANT SAPONINS
653 $aMASS SPECTROMETRY
653 $aMETHYLATION ANALYSIS
653 $aMONOSACCHARIDE ANALYIS
653 $aQUILLAJA BRASILIENSIS
700 1 $aBENNADJI, Z.
700 1 $aFERREIRA, F.
700 1 $aOLIVARO, C.
773 $tPhytochemical Analysis, 1 November 2019, Volume 30, Issue 6, Pages 644-652. Doi: https://doi.org/10.1002/pca.2837
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| 101. |  | MONTOSSI, F.; DE BARBIERI, I.; CIAPPESONI, G.; SOARES DE LIMA, J.M.; GRATTAROLA, M.; PÉREZ JONES, J.; DONAGARAY, F.; FROS, A. Desde el campo experimental al desarrollo del agronegocio lanero proyecto merino fino del Uruguay Revista INIA Uruguay, 2010, no. 21 p. 10-15 (Revista INIA; 21)| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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| 102. | | MONTOSSI, F.; DE BARBIERI, I.; CIAPPESONI, G.; SOARES DE LIMA, J.M.; GRATTAROLA, M.; PÉREZ JONES, J.; DONAGARAY, F.; FROS, A. Proyecto merino fino del Uruguay: desde el campo experimental al desarrollo del agronegocio lanero. ln: INIA Tacuarembó. Unidad Experimental Glencoe. Día de campo, Glencoe, 10 setiembre 2010, Paysandú, Uruguay. Pasturas y producción animal. Tacuarembó (Uruguay): INIA, 2010. p. 27-31 (INIA Serie Actividades de Difusión ; 619) INIA| Biblioteca(s): INIA Tacuarembó. |
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| 103. | | MONTOSSI, F.; DE BARBIERI, I.; CIAPPESONI, G.; SOARES DE LIMA, J.M.; GRATTAROLA, M.; PÉREZ JONES, J.; DONAGARAY, F.; FROS, A. Proyecto Merino Fino del Uruguay: Una experiencia exitosa de la investigación nacional que culminó en el desarrollo un negocio lanero. Asociacción Argentina Criadores de Merino, Anuario 2010, p. 66-73.| Biblioteca(s): INIA Tacuarembó. |
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| 105. | | MONTOSSI, F.; DE BARBIERI, I.; MEDEROS, A.; CIAPPESONI, G.; LUZARDO, S.; RAVAGNOLO, O.; SOARES DE LIMA, J.M.; SILVEIRA, C.; PLATERO, P.; NOLLA, M.; GRATTAROLA, M.; GIMENO, D.; PÉREZ JONES, J.; FROS, A. Proyecto Merino Fino del Uruguay: Fase I. ln: INIA TACUAREMBÓ. ESTACIÓN EXPERIMENTAL GLENCOE. Día de campo. Producción animal, pasturas. Estación Experimental Glencoe, Paysandú, 14 noviembre, 2006. Tacuarembó (Uruguay): INIA, 2006. (INIA Serie Actividades de Difusión ; 473) Personal de apoyo: Julio Frugoni, Homero Martínez, Fernando Rovira, Daniel Bottero, Juan Levratto, Mauro Bentancur, P.ablo Cuadro, Wilfredo Zamit, Héctor Rodríguez, Luis Eduardo Moreira, Liria Silva y Hildo González.| Biblioteca(s): INIA Tacuarembó. |
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| 106. | | MONTOSSI, F.; DE BARBIERI, I.; MEDEROS, A.; CIAPPESONI, G.; LUZARDO, S.; RAVAGNOLO, O.; SOARES DE LIMA, J.M.; SILVEIRA, C.; RODRIGUEZ, A.; GRATTAROLA, M.; GIMENO, D.; PÉREZ JONES, J.; FROS, A. Proyecto Merino Fino del Uruguay. Fase I. Tendencias genéticas y producción y valorización de lanas. In: INIA Tacuarembó. Unidad Experimental Glencoe. Día de campo, 24 octubre, Paysandú, 2007. Alternativas de intensificación, especialización, diversificación y valorización de la ganadería ovina y bovina en el Basalto. Tacuarembó (Uruguay): INIA, 2007. p. 5-7 (INIA Serie Actividades de Difusión ; 518)| Biblioteca(s): INIA Tacuarembó. |
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| 107. | | MONTOSSI, F.; FERNANDEZ, E.; SOARES DE LIMA, J.M.; BANCHERO, G.; TIERI, M.; LA MANNA, A.; MIERES, J. En tiempos de agricultura: ¿Hacia dónde vamos en la invernada intensiva? Revista INIA Uruguay, 2011, no. 27, p. 19-24 (Revista INIA; 27)| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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| 119. | | MONTOSSI, F.; SOARES DE LIMA, J.M.; BRITO, G.; CAZZULI, F.; BERRETTA, E. Productivity and economic impact of the diferent technological strategies designed for extensive livestock production of the basaltic region of Uruguay: cow-calf subsystem. In: International Conference: Steps to Sustainable Livestock, University of Bristol, England, UK, 12th-15th January, 2016. Abstract. p. 94.| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Tacuarembó. |
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| 120. | | MONTOSSI, F.; SOARES DE LIMA, J.M.; BRITO, G.; CAZZULI, F.; BERRETTA, E. Productivity impact of the different technological strategies designed for livestock production in the basaltic region of Uruguay: rearing and fattening sub systems. In: International Conference: Steps to Sustainable Livestock, University of Bristol, England, UK, 12th-15th January, 2016. Abstract. p. 95.| Tipo: Abstracts/Resúmenes |
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